The conservation of biodiversity has been a recurring theme of studies and debates carried out by the scientific community and governmental institutions. In this context, many efforts are directed towards the maintenance of Brazilian natural resources. Given the above, the main objective of this research is to discuss the structure of the Ecological-Economic Macro Zoning in the Legal Amazon in Brazil. With the adoption of multiple methods - exploratory, descriptive and analytic - this study is based on secondary sources interpretation and geoprocessing techniques. Based on territorial units, three typologies of territory were delimited: Network - focused on integration with neighboring spaces and stimulation of alternative means of production; Frontier - intended to contain the agricultural expansion; and Zoning - determined for conservation of natural resources. The proposed administration strategies consider contemporary themes such as climate change, maintenance of environmental services and encouragement of technology, among others. In this way, it is concluded that the established guidelines are innovative and can support the development of other ecological-economic zoning.