Our study aimed to investigate the influence of S supplementation on biochemical and physiological tools of soybean plants subjected to drought stress, analyzing the accumulation of photosynthetic pigments, carbohydrates and free amino acids. The experimental design was in randomized blocks (DBC), in a 3 × 2 × 2 factorial arrangement, consisting of three levels of drought stress (60% field capacity (FC) - control treatment; 45% FC - moderated drought; and 30% FC - severe drought), two doses of sulfur (S) (40 and 80 kg ha-1,) and two soybean genotypes (BÔNUS 8579 and M8808), with four replications. Were the quantification of photosynthetic pigments, chlorophyll a, b and total, carotenoids, total soluble carbohydrates and free amino acids. In conclusion, our data clearly evidence that S supplementation at 80 kg ha-¹ promotes important changes in photosynthetic pigments and organic solutes compounds. The main alterations were registered in M8808 genotype under serve water deficit.